Fachgebiet Tierzüchtung, Institut für Tierhaltung und Tierzüchtung (470), Universität Hohenheim, Garbenstr. 17, D-70593, Stuttgart, Germany.
Theor Appl Genet. 1994 Sep;89(1):116-20. doi: 10.1007/BF00226992.
In order to identify DNA variants within the 5'-flanking region of the bovine κ-casein (κCn)-encoding gene, this area of the gene from 13 cows belonging to seven breeds (Holstein Friesian, Brown Swiss, German Simmental, Jersey, Galloway, Scottish Highland and Ceylon Dwarf Zebu) was analysed. For each individual, about 1 kb of the 5'-flanking region including exon I was amplified by polymerase chain reaction (PCR). The biotinylated PCR product was immobilized on magnetic beads followed by direct bidirectional sequencing using an automated DNA sequencer. Fifteen DNA variants were identified, some of which are located within potential regulatory sites and possibly involved in the expression of the κ-casein encoding gene.
为了鉴定牛κ-酪蛋白（κCn）编码基因 5'-侧翼区的 DNA 变异，对来自 7 个品种（荷斯坦-弗里生、瑞士褐牛、德国西门塔尔牛、泽西牛、盖洛韦牛、苏格兰高地牛和锡兰矮种黄牛）的 13 头奶牛的该基因区域进行了分析。对每个个体，通过聚合酶链反应（PCR）扩增约 1kb 的包括外显子 I 的 5'-侧翼区。生物素化的 PCR 产物固定在磁珠上，然后使用自动 DNA 测序仪进行直接双向测序。鉴定出 15 种 DNA 变异，其中一些位于潜在的调控位点内，可能与 κ-酪蛋白编码基因的表达有关。
