Sato H, Ishii T, Sugita Y, Tateishi N, Bannai S
Department of Biochemistry, Tsukuba University Medical School, Ibaraki, Japan.
Biochim Biophys Acta. 1993 May 14;1148(1):127-32. doi: 10.1016/0005-2736(93)90169-z.
The synthesis of 23 kDa protein was enhanced when mouse peritoneal macrophages were exposed to oxidative agents such as hydrogen peroxide and menadione, or to sulfhydryl-reactive agents such as diethylmaleate, cadmium chloride and sodium arsenite. After 11 h exposure to these agents the 23 kDa protein was one of the actively synthesized proteins in the macrophages. Under similar conditions the 34 kDa protein previously identified as heme oxygenase, was induced and its synthesis preceded that of the 23 kDa protein. Neither the 23 kDa or the 34 kDa protein was induced by hyperthermia. Conversely, the various oxidative and sulfhydryl-reactive agents employed here did not induce the major heat shock proteins in the macrophages. When the macrophages were activated by bacterial lipopolysaccharide or other stimulants, many proteins are known to be induced, however, the 23 kDa and 34 kDa proteins were not induced. The 34 kDa protein, i.e., heme oxygenase, has been found to be stress-induced in various types of cell, but not the 23 kDa protein. This suggests that the 23 kDa protein is a stress protein predominantly expressed in macrophages.
当小鼠腹腔巨噬细胞暴露于过氧化氢和甲萘醌等氧化剂，或马来酸二乙酯、氯化镉和亚砷酸钠等巯基反应剂时，23 kDa蛋白的合成会增强。在暴露于这些试剂11小时后，23 kDa蛋白是巨噬细胞中活跃合成的蛋白之一。在类似条件下，先前鉴定为血红素加氧酶的34 kDa蛋白被诱导，其合成先于23 kDa蛋白。23 kDa或34 kDa蛋白均未被热疗诱导。相反，这里使用的各种氧化剂和巯基反应剂均未诱导巨噬细胞中的主要热休克蛋白。当巨噬细胞被细菌脂多糖或其他刺激物激活时，已知会诱导许多蛋白，但23 kDa和34 kDa蛋白未被诱导。34 kDa蛋白，即血红素加氧酶，已发现在各种类型的细胞中可被应激诱导，但23 kDa蛋白则不然。这表明23 kDa蛋白是主要在巨噬细胞中表达的应激蛋白。
