Fumoux F, Guigou V, Blaise D, Maraninchi D, Fougereau M, Schiff C
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
Blood. 1993 Jun 1;81(11):3153-7.
Reconstitution of the human Ig repertoire after allogeneic and autologous bone marrow transplantation (BMT) was followed by analysis of the expression of the six VH families using in situ hybridization on BM cells at 30, 60, and 90 days postgraft. Our results indicate that during the early post-transplantation period, the expressed repertoire of VH is dramatically affected: VH3, the major expressed family in adult B cells, is decreased two- to threefold and is compensated by transient overexpression of the other families, especially VH4, VH5, and VH6. Similar results were observed in allogeneic and autologous grafted patients. Kinetics of reconstitution mimics normal repertoire development described in ontogeny, although normalization, as compared with the adult pattern, may take as long as 1 year.
在异基因和自体骨髓移植（BMT）后对人类免疫球蛋白重链（Ig）库进行重建，然后在移植后30天、60天和90天使用原位杂交技术分析骨髓细胞中六个重链可变区（VH）家族的表达情况。我们的结果表明，在移植后的早期阶段，VH的表达库受到显著影响：VH3是成年B细胞中主要表达的家族，其表达量降低了两到三倍，并由其他家族尤其是VH4、VH5和VH6的短暂过表达所补偿。在异基因和自体移植患者中均观察到类似结果。重建动力学模拟了个体发育中描述的正常库发育情况，尽管与成年模式相比，正常化可能需要长达1年的时间。
