Gaitan E, Cooksey R C, Legan J, Cruse J M, Lindsay R H, Hill J
University of Mississippi School of Medicine, Jackson.
Thyroid. 1993 Spring;3(1):49-53. doi: 10.1089/thy.1993.3.49.
Goiter in iodine-sufficient areas has been linked to water-borne goitrogens in watersheds and aquifers rich in coal and shale. In the present study, the potential antithyroid and goitrogenic effects of coal-water extracts (CWE) were investigated in vivo in rats after chronic and acute oral administration of CWE, and in vitro by a thyroid peroxidase (TPO) enzyme system. CWE was prepared by continuous extraction of ground (40 mesh) Appalachian coal with goitrogen-free water (GFW). Female Buffalo rats fed on Purina iodine-rich diet (12 micrograms I-/day/rat), were given ad lib CWE (50 mg/ml; approximately 20 mL/day/rat) or GFW (controls) for 2 months. At the end of the experiment, 125I 1 microCi, was injected i.p. and 4 h later the thyroid glands were removed, weighed, and analyzed histologically and for total 125I and 125I-labeled compounds. Rats on CWE had larger thyroid glands [7.2 +/- 0.3 mg/100 g (mean +/- SE) vs 5.0 +/- 0.5 controls; p < 0.005] with distinct histological changes of smaller thyroid follicles, some with columnar epithelium, and with more dense colloid than in controls, and had significant inhibition of the coupling mechanism for production of thyroid hormones [125MIT + DIT/125T3 + T4: 5.1 +/- 0.2 vs 3.9 +/- 0.1 controls, p < 0.005; and 125T3 + T4 (%): 10.6 +/- 0.3 vs 12.6 +/- 0.4 controls, p < 0.005]. Female Sprague-Dawley rats under the same conditions as Buffalo rats were given acutely by GI tube 2 mL of CWE (5 g/mL) or GFW (controls).(ABSTRACT TRUNCATED AT 250 WORDS)
在碘充足地区，甲状腺肿与富含煤和页岩的流域及含水层中与水有关的致甲状腺肿物质有关。在本研究中，通过对大鼠进行慢性和急性口服煤水提取物（CWE）来研究其体内潜在的抗甲状腺和致甲状腺肿作用，并通过甲状腺过氧化物酶（TPO）酶系统进行体外研究。CWE通过用无致甲状腺肿物质的水（GFW）连续提取研磨成40目的阿巴拉契亚煤来制备。以普瑞纳富碘饮食（12微克碘/天/只大鼠）喂养的雌性布法罗大鼠，随意给予CWE（50毫克/毫升；约20毫升/天/只大鼠）或GFW（对照组），持续2个月。实验结束时，腹腔注射1微居里的125I，4小时后取出甲状腺，称重，并进行组织学分析以及检测总125I和125I标记化合物。接受CWE的大鼠甲状腺更大[7.2±0.3毫克/100克（平均值±标准误），对照组为5.0±0.5；p<0.005]，甲状腺滤泡明显变小，一些有柱状上皮，胶体比对照组更致密，有明显的组织学变化，并且甲状腺激素产生的偶联机制受到显著抑制[125MIT+DIT/125T3+T4：5.1±0.2，对照组为3.9±0.1，p<0.005；125T3+T4（%）：10.6±0.3，对照组为12.6±0.4，p<0.005]。在与布法罗大鼠相同的条件下，通过胃管给雌性斯普拉格 - 道利大鼠急性给予2毫升CWE（5克/毫升）或GFW（对照组）。（摘要截短于250字）
