Birkholz S, Knipp U, Nietzki C, Adamek R J, Opferkuch W
Department of Medical Microbiology and Immunology, Ruhr-Universität, Bochum, FRG.
FEMS Immunol Med Microbiol. 1993 Apr;6(4):317-24. doi: 10.1111/j.1574-695X.1993.tb00344.x.
Lipopolysaccharide of Helicobacter pylori was tested for its mitogenicity and for its ability to stimulate cytokine release in human peripheral blood mononuclear cells (PBMC) of healthy and H. pylori-infected blood donors. Mitogenicity in PBMC induced by H. pylori LPS was similar to that induced by Campylobacter jejuni lipopolysaccharide, but lower than that induced by Escherichia coli lipopolysaccharide in the H. pylori negative blood donor group. Furthermore, H. pylori LPS was able to induce tumour necrosis factor (TNF) interleukin 1 (IL-1) and interleukin 6 (IL-6) secretion of PBMC. Compared with the ability of C. jejuni and E. coli lipopolysaccharides to stimulate cytokine release, H. pylori lipopolysaccharide induced a significantly lower TNF and IL-1 secretion of PBMC than the other tested bacterial lipopolysaccharides. Similar amounts of IL-6 release were obtained by stimulation of PBMC with H. pylori and C. jejuni lipopolysaccharides, whereas a higher IL-6 release was measured by stimulation with E. coli lipopolysaccharide. The results of this study suggest that H. pylori lipopolysaccharide has a lower immunological activity than lipopolysaccharides of other intestinal bacteria. This is probably due to its unusual acylation and phosphorylation pattern of lipid A.
对幽门螺杆菌的脂多糖进行了检测，评估其在健康和幽门螺杆菌感染的献血者外周血单核细胞（PBMC）中的促有丝分裂活性以及刺激细胞因子释放的能力。在幽门螺杆菌阴性献血者组中，幽门螺杆菌脂多糖诱导PBMC的促有丝分裂活性与空肠弯曲菌脂多糖诱导的相似，但低于大肠杆菌脂多糖诱导的。此外，幽门螺杆菌脂多糖能够诱导PBMC分泌肿瘤坏死因子（TNF）、白细胞介素1（IL-1）和白细胞介素6（IL-6）。与空肠弯曲菌和大肠杆菌脂多糖刺激细胞因子释放的能力相比，幽门螺杆菌脂多糖诱导PBMC分泌的TNF和IL-1显著低于其他受试细菌脂多糖。用幽门螺杆菌和空肠弯曲菌脂多糖刺激PBMC获得的IL-6释放量相似，而用大肠杆菌脂多糖刺激测得的IL-6释放量更高。本研究结果表明，幽门螺杆菌脂多糖的免疫活性低于其他肠道细菌的脂多糖。这可能是由于其脂质A不寻常的酰化和磷酸化模式。
