Llovo J, Lopez A, Fabregas J, Muñoz A
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago, Santiago de Compostela, Spain.
J Infect Dis. 1993 Jun;167(6):1477-80. doi: 10.1093/infdis/167.6.1477.
Cell surface carbohydrates from four clinical isolates of Cryptosporidium parvum were analyzed by agglutination assays using a battery of 20 highly purified lectins with affinity for receptor molecules containing N-acetyl-D-glucosamine (GlcNAc), N-acetyl-D-galactosamine, galactose, mannose, glucose, fucose, and N-acetyl-neuraminic acid. Tomentine, a lectin from the green seaweed Codium tomentosum, and UEA-II lectin, from Ulex europeus, both of them GlcNAc-specific lectins, agglutinated the oocysts. Subsequent inhibition assay confirmed the presence of this sugar on the surface of Cryptosporidium parvum oocysts. Codium fragile lectin, from another green seaweed, also exhibited agglutination activity against the oocysts. This is the first published demonstration of such an interaction between a human coccidian and lectins from seaweeds.
使用一系列20种对含有N-乙酰-D-葡萄糖胺（GlcNAc）、N-乙酰-D-半乳糖胺、半乳糖、甘露糖、葡萄糖、岩藻糖和N-乙酰神经氨酸的受体分子具有亲和力的高度纯化凝集素，通过凝集试验分析了来自4株微小隐孢子虫临床分离株的细胞表面碳水化合物。来自绿海藻绒毛仙掌藻的凝集素托门汀和来自欧洲荆豆的UEA-II凝集素，这两种都是GlcNAc特异性凝集素，能够凝集卵囊。随后的抑制试验证实了这种糖存在于微小隐孢子虫卵囊表面。来自另一种绿海藻的脆弱仙掌藻凝集素也表现出对卵囊的凝集活性。这是首次发表的关于人类球虫与海藻凝集素之间这种相互作用的证明。
