Okada K, Funahashi M, Iseki K, Ishii Y
Second Department of Ophthalmology, Toho University School of Medicine, Tokyo, Japan.
J Cataract Refract Surg. 1993 May;19(3):431-4. doi: 10.1016/s0886-3350(13)80321-5.
We developed a method to evaluate cell adherence to different intraocular lens (IOL) materials in one eye in which we coated one half of the anterior surface of a poly(methyl methacrylate) (PMMA) IOL with poly(dimethyl siloxane), a silicone material. The lenses were implanted in the anterior chamber of rabbit eyes without lens extraction. Postoperative cellular reaction on the IOL surfaces was studied by specular microscopy. The IOLs were fixed in situ, removed from the eyes, and stained with hematoxylin-eosin so we could evaluate the cell population on the silicone and PMMA surface. Fewer cells were scattered on the silicone surface than on the PMMA surface. This method excludes surgical effects and allows a comparison of various IOL materials in the same eye.
我们开发了一种方法，用于评估一只眼睛中细胞对不同人工晶状体（IOL）材料的粘附情况。在该方法中，我们用聚二甲基硅氧烷（一种硅酮材料）涂覆了聚甲基丙烯酸甲酯（PMMA）人工晶状体前表面的一半。这些晶状体被植入兔眼的前房，未进行晶状体摘除。通过镜面显微镜研究了人工晶状体表面的术后细胞反应。将人工晶状体原位固定，从眼中取出，并用苏木精-伊红染色，以便我们能够评估硅酮和PMMA表面的细胞数量。与PMMA表面相比，硅酮表面上散落的细胞较少。该方法排除了手术影响，并允许在同一只眼睛中比较各种人工晶状体材料。
