Noguchi J, Yoshida M, Ikadai H, Imamichi T, Watanabe G, Taya K
Imamichi Institute for Animal Reproduction, Dejima, Ibaraki, Japan.
J Reprod Fertil. 1993 Mar;97(2):433-9. doi: 10.1530/jrf.0.0970433.
A new rat mutant showing aspermia was investigated. Groups of 4-7 mutant male rats were killed at 3, 5, 10, 15 and 25 weeks of age. Examination by microscope showed apparent abnormalities in the seminiferous epithelium from 3 weeks of age onward. Inclusion-like bodies were observed in the cytoplasm of pachytene spermatocytes from 3 weeks old and instead of spermiation, polynuclear giant cells were formed within the seminiferous epithelium after 5 weeks of age. Histological analysis of seminiferous epithelium of adult mutant rats also showed a marked decrease in the number of preleptotene, leptotene and pachytene spermatocytes and tubules containing only spermatogonia and Sertoli cells in the seminiferous epithelium increased with age. However, the combination of other cellular elements of germ cells in the seminiferous epithelium was similar to that in normal rats and the distribution rate of these seminiferous tubules was close to that of normal rats, indicating that cyclicity of seminiferous epithelium was still maintained in the mutant rats despite the lack of spermiation. Plasma concentrations of FSH and LH were significantly higher in the mutant male rats than in normal male rats at 5 and 10 weeks of age onward, respectively. Plasma concentrations of testosterone were lower in the mutant male rats than in normal male rats. Silastic capsules containing testosterone were implanted into the unilateral testis of adult mutant male rats and animals were autopsied 5 weeks later. However, intratesticular administration of testosterone did not affect restoration of spermatogenesis.(ABSTRACT TRUNCATED AT 250 WORDS)
对一只表现为无精子症的新型大鼠突变体进行了研究。将4至7只突变雄性大鼠分为一组，分别在3周、5周、10周、15周和25周龄时处死。显微镜检查显示，从3周龄起，生精上皮就出现了明显异常。在3周龄的粗线期精母细胞胞质中观察到包涵体样结构，5周龄后，生精上皮内未出现精子释放，而是形成了多核巨细胞。成年突变大鼠生精上皮的组织学分析还显示，前细线期、细线期和粗线期精母细胞数量显著减少，且生精上皮中仅含有精原细胞和支持细胞的小管数量随年龄增加而增多。然而，生精上皮中生殖细胞的其他细胞成分组合与正常大鼠相似，这些生精小管的分布率也与正常大鼠相近，这表明尽管缺乏精子释放，突变大鼠的生精上皮仍保持周期性。分别在5周龄和10周龄起，突变雄性大鼠血浆中促卵泡激素（FSH）和促黄体生成素（LH）的浓度显著高于正常雄性大鼠。突变雄性大鼠血浆中睾酮的浓度低于正常雄性大鼠。将含有睾酮的硅橡胶胶囊植入成年突变雄性大鼠的单侧睾丸，5周后对动物进行解剖。然而，睾丸内给予睾酮并未影响精子发生的恢复。（摘要截断于250字）
