Atagi Y, Ikadai H, Kurohmaru M, Hayashi Y
Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
J Vet Med Sci. 1993 Apr;55(2):301-6. doi: 10.1292/jvms.55.301.
The morphology of the testis of the mutant As (aspermia) strain rat with severely interrupted spermatogenesis was investigated in the present study. The most advanced development in spermatogenesis was observed at the step 8 at which spermatids had basally-oriented acrosomes. Multinucleate giant cells derived from round spermatids were frequently encountered within the seminiferous tubules. The functionally normal status of the blood-testis barrier between adjacent Sertoli cells was confirmed by morphological and lanthanum tracer studies. By light microscopy, a peculiar structure was found within the pachytene spermatocyte, consisting of numerous particles of approximately 25 nm in diameter. By histochemical and electron microscopic studies, this structure was identified as an aggregate of ribosomes. Neither immature spermatogenic cells up to the pachytene phase, nor Sertoli cells nor interstitial regions showed a recognizable abnormality. It seems that the testicular disruption in the As mutant rat is due to the abnormality in the protein synthetic pathway in pachytene spermatocytes.
本研究对精子发生严重受阻的突变型无精子症（As）品系大鼠的睾丸形态进行了研究。精子发生的最先进阶段出现在第8步，此时精子细胞的顶体位于基部。在生精小管内经常能见到由圆形精子细胞衍生而来的多核巨细胞。通过形态学和镧示踪研究证实了相邻支持细胞之间血睾屏障的功能正常状态。通过光学显微镜观察，在粗线期精母细胞内发现了一种特殊结构，由许多直径约25纳米的颗粒组成。通过组织化学和电子显微镜研究，该结构被鉴定为核糖体聚集体。直至粗线期的未成熟生精细胞、支持细胞或间质区域均未显示出可识别的异常。看来As突变大鼠的睾丸破坏是由于粗线期精母细胞蛋白质合成途径的异常所致。
