Giralt M, Lafuente A, Pujol F, Mallol J
Unit of Pharmacology, School of Medicine at Reus, University of Barcelona, Spain.
J Urol. 1993 Jun;149(6):1452-4. doi: 10.1016/s0022-5347(17)36413-3.
Glutathione content and glutathione S-transferase activity have been studied in human bladder specimens obtained from controls and from patients with superficial transitional cell carcinoma (tumor samples and peri-tumor normal tissues from the same patient). After combining an earlier study from our laboratory with the additional material presented (9 healthy controls and 25 transitional cell carcinoma patients), it can be observed that glutathione S-transferase activity was significantly greater in tumor than in peri-tumor normal tissue (34 patients, p < 1 x 10(-7)) or in normal mucosa (17 controls, p < 1 x 10(-3)). Glutathione content was significantly greater in tumor than in peri-tumor normal tissue (p < 5 x 10(-3)) or in normal mucosa (p < 2 x 10(-2)), with this increase being evident only in smokers. When comparing normal mucosa and peri-tumor samples no significant differences were found either for glutathione S-transferase activity or for glutathione content. Results demonstrate the relationship between the glutathione S-transferase/glutathione system and development of transitional cell carcinoma, as well as its role in cellular resistance to chemotherapy.
对取自对照组以及浅表性移行细胞癌患者（同一患者的肿瘤样本和肿瘤周围正常组织）的人体膀胱标本中的谷胱甘肽含量和谷胱甘肽S -转移酶活性进行了研究。将我们实验室早期的一项研究与本文提供的额外材料（9名健康对照者和25名移行细胞癌患者）相结合后可以观察到，谷胱甘肽S -转移酶活性在肿瘤组织中显著高于肿瘤周围正常组织（34例患者，p < 1×10⁻⁷）或正常黏膜组织（17例对照者，p < 1×10⁻³）。谷胱甘肽含量在肿瘤组织中显著高于肿瘤周围正常组织（p < 5×10⁻³）或正常黏膜组织（p < 2×10⁻²），且这种增加仅在吸烟者中明显。比较正常黏膜和肿瘤周围样本时，谷胱甘肽S -转移酶活性和谷胱甘肽含量均未发现显著差异。结果证明了谷胱甘肽S -转移酶/谷胱甘肽系统与移行细胞癌发生之间的关系，以及其在细胞对化疗的抗性中的作用。
