通过免疫电子显微镜观察DNA中紫外线诱导的胸腺嘧啶二聚体
Visualization of ultraviolet light-induced thymine dimers in DNA by immunoelectron microscopy.
作者信息
Udono M U, Hori M, Hirayama M, Yoshida H, Suematsu T, Suematsu T, Mori T, Matsunaga T, Mizuno T, Nikaido O
机构信息
Department of Dermatology, Nagasaki University School of Medicine, Japan.
出版信息
Photochem Photobiol. 1993 Apr;57(4):752-4. doi: 10.1111/j.1751-1097.1993.tb02949.x.
To see the damage of DNA due to ultraviolet-B more distinctly, immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. As a result, we could detect the existence of thymine dimers on human genomic DNA and pUC18 plasmid DNA visually. This technique can be useful to locate the photoproducts formed on DNA.
为了更清楚地观察紫外线B对DNA的损伤，我们使用了一种针对环丁烷型胸腺嘧啶二聚体的单克隆抗体进行免疫电子显微镜研究。结果，我们能够直观地检测到人类基因组DNA和pUC18质粒DNA上胸腺嘧啶二聚体的存在。这项技术对于定位DNA上形成的光产物可能会很有用。
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