Anthone G J, Barry M K, Yeo C J
Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland.
Am J Surg. 1993 Jun;165(6):704-7. doi: 10.1016/s0002-9610(05)80792-5.
Ileal water and electrolyte absorption exceed jejunal absorption in both the basal and meal-stimulated states. The purposes of these experiments were to determine: (1) if luminal bile acids alter basal or meal-stimulated intestinal absorption, and (2) if there is site specificity or meal stimulation of intestinal bile acid absorption. Twenty-five centimeters of canine proximal jejunal and distal ileal Thiry-Vella fistulas were constructed. Simultaneous jejunal and ileal absorption studies (n = 88) were performed with a luminal perfusate containing polyethylene glycol labeled with radioactive carbon-14 to calculate the absorption of water, electrolytes, and the bile acid taurocholate (TC). In group 1, there was no TC in the luminal perfusate, whereas in group 2, 10 mM of TC was present in the luminal perfusate. Half of the observations were performed after a meal stimulus, which consisted of an orally ingested, 480-kcal mixed nutrient meal. Intraluminal TC did not affect basal or meal-stimulated water or electrolyte absorption. In both the basal and meal-stimulated states, ileal absorption of water, electrolytes, and TC significantly exceeded jejunal absorption (p < 0.05). A meal significantly stimulated water and electrolyte absorption in both the jejunum and ileum, but it stimulated absorption of TC in the ileum only (p < 0.05). Intraluminal TC does not alter basal or meal-stimulated intestinal water and electrolyte absorption. A meal stimulates increased water and electrolyte absorption in both the jejunum and the ileum, but it stimulates bile acid absorption in the ileum only. Bile acid absorption is site specific and responsive to a meal stimulus.
在基础状态和进餐刺激状态下,回肠对水和电解质的吸收均超过空肠。这些实验的目的是确定:(1)肠腔内胆汁酸是否会改变基础状态或进餐刺激状态下的肠道吸收;(2)肠道胆汁酸吸收是否存在部位特异性或对进餐刺激有反应。构建了25厘米长的犬近端空肠和远端回肠的Thiry-Vella瘘管。使用含有用放射性碳-14标记的聚乙二醇的肠腔灌注液进行同步空肠和回肠吸收研究(n = 88),以计算水、电解质和胆汁酸牛磺胆酸盐(TC)的吸收情况。在第1组中,肠腔灌注液中没有TC,而在第2组中,肠腔灌注液中存在10 mM的TC。一半的观察是在进餐刺激后进行的,进餐刺激包括口服一顿480千卡的混合营养餐。肠腔内TC不影响基础状态或进餐刺激状态下的水或电解质吸收。在基础状态和进餐刺激状态下,回肠对水、电解质和TC的吸收均显著超过空肠(p < 0.05)。进餐显著刺激了空肠和回肠中的水和电解质吸收,但仅刺激了回肠中TC的吸收(p < 0.05)。肠腔内TC不会改变基础状态或进餐刺激状态下的肠道水和电解质吸收。进餐刺激空肠和回肠中水和电解质吸收增加,但仅刺激回肠中胆汁酸吸收。胆汁酸吸收具有部位特异性且对进餐刺激有反应。