Ruby E G, Nealson K H
Science. 1977 Apr 22;196(4288):432-4. doi: 10.1126/science.850787.
A strain of Photobacterium fischeri that emits yellow light has been isolated from seawater. The bimodal spectrum, which is unique among the luminous bacteria, consists of a major band with a maximum at 545 nanometers and a minor band with a maximum at 500 nanometers. The former represents a heretofore unreported range of emission for luminous bacteria, while the latter coincides with the emission spectrum of typical blue-greeen-emitting strains of P. fischeri. The relative contributions of these two bands to the total in vivo luminescence changes as a function of ambient temperature. When luciferase is extracted and luminescence observed in vitro, the emission is entirely blue-green, identical with that of luciferase isolated from other strains of P. fischeri.
从海水中分离出了一种能发出黄色光的费氏弧菌菌株。这种双峰光谱在发光细菌中是独一无二的，它由一个在545纳米处有最大值的主带和一个在500纳米处有最大值的次带组成。前者代表了迄今为止发光细菌未报道过的发射范围，而后者与典型的发出蓝绿色光的费氏弧菌菌株的发射光谱一致。这两个波段对体内总发光的相对贡献随环境温度而变化。当提取荧光素酶并在体外观察发光时，发射光完全是蓝绿色的，与从其他费氏弧菌菌株中分离出的荧光素酶相同。
