Kempner E S, Hanson F E
J Bacteriol. 1968 Mar;95(3):975-9. doi: 10.1128/jb.95.3.975-979.1968.
Studies of luminescence in growing cultures of Photobacterium fischeri revealed the characteristic kinetics of light emission, including a minimal phase of bacterial light output. A dialyzable substance present in the nutrient broth medium caused this transient inhibition in light production, although this substance did not affect culture growth. Experiments were carried out to determine the mechanism of action and the chemical properties of the inhibitor. The results suggest that the inhibitor may be binding directly to the luciferase molecules.
对费氏弧菌生长培养物中的发光现象进行的研究揭示了发光的特征动力学,包括细菌光输出的一个最小阶段。尽管营养肉汤培养基中存在的一种可透析物质不会影响培养物生长,但它会导致光产生的这种短暂抑制。开展了实验以确定该抑制剂的作用机制和化学性质。结果表明,该抑制剂可能直接与荧光素酶分子结合。