Fang Y I, Komatsu Y, Momose K
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
J Smooth Muscle Res. 1993 Feb;29(1):9-17. doi: 10.1540/jsmr.29.9.
Cells of guinea pig ileal longitudinal muscle layer were dispersed by treating the minced tissue with highly purified collagenase and papain. The cells were spindle shaped, 100 to 150 microns long and 5 to 10 microns wide. Contractility of the dispersed cells was determined by "cell adhesion method" resulting that the cells were contracted by carbachol in a dose-dependent manner, but sensitivity and degree of the contraction were different in each cell. Binding assay method resulted that Kd values did not change by treatment with collagenase and papain. These results indicate that contractility of each cell in the tissue is originally different and dispersion of the cells does not affect on the cell contractility.
用高度纯化的胶原酶和木瓜蛋白酶处理豚鼠回肠纵肌层的切碎组织，使其细胞分散。这些细胞呈纺锤形，长100至150微米，宽5至10微米。采用“细胞黏附法”测定分散细胞的收缩性，结果表明，卡巴胆碱可使细胞呈剂量依赖性收缩，但每个细胞的收缩敏感性和程度不同。结合测定法结果显示，用胶原酶和木瓜蛋白酶处理后，解离常数（Kd）值没有变化。这些结果表明，组织中每个细胞的收缩性原本就不同，细胞分散不会影响细胞的收缩性。
