Nakayama S, Kretsinger R H
Department of Biology, University of Virginia, Charlottesville 22901.
J Mol Evol. 1993 May;36(5):458-76. doi: 10.1007/BF02406722.
In the first report in this series we presented dendrograms based on 152 individual proteins of the EF-hand family. In the second we used sequences from 228 proteins, containing 835 domains, and showed that eight of the 29 subfamilies are congruent and that the EF-hand domains of the remaining 21 subfamilies have diverse evolutionary histories. In this study we have computed dendrograms within and among the EF-hand subfamilies using the encoding DNA sequences. In most instances the dendrograms based on protein and on DNA sequences are very similar. Significant differences between protein and DNA trees for calmodulin remain unexplained. In our fourth report we evaluate the sequences and the distribution of introns within the EF-hand family and conclude that exon shuffling did not play a significant role in its evolution.
在本系列的第一篇报告中，我们展示了基于EF手型家族152种个体蛋白质的系统树图。在第二篇报告中，我们使用了来自228种蛋白质的序列，其中包含835个结构域，并表明29个亚家族中的8个是一致的，其余21个亚家族的EF手型结构域具有不同的进化历史。在本研究中，我们使用编码DNA序列计算了EF手型亚家族内部和之间的系统树图。在大多数情况下，基于蛋白质和DNA序列的系统树图非常相似。钙调蛋白的蛋白质树和DNA树之间的显著差异仍无法解释。在我们的第四篇报告中，我们评估了EF手型家族内的序列和内含子分布，并得出结论，外显子重排在其进化中没有发挥重要作用。
