Stein J P, Munjaal R P, Lagace L, Lai E C, O'Malley B W, Means A R
Proc Natl Acad Sci U S A. 1983 Nov;80(21):6485-9. doi: 10.1073/pnas.80.21.6485.
An eel calmodulin cDNA probe has been used to isolate a calmodulin gene from a chicken DNA library. Sequence analysis revealed this calmodulin gene (cCM1) to contain the nucleotides that code for 148 amino acids, a termination codon, and 486 residues of 3'-noncoding sequence before an A-A-T-A-A-A poly(A) addition signal. The amino acid sequence derived from these nucleotides is 87% homologous to that of bovine brain calmodulin. cCM1 is one of two calmodulin genes in the chicken genome but is unique in that it does not contain intervening sequences to interrupt the structural segments of the protein. This suggests that cCM1 originated as a processed gene copy derived from the other calmodulin gene, cCL1, a circumstance usually associated with pseudogenes. In contrast, cCM1 appears to be a functional member of a multigene family whose expression is specific for muscle cells.
用鳗鱼钙调蛋白cDNA探针从鸡DNA文库中分离出一个钙调蛋白基因。序列分析表明，这个钙调蛋白基因（cCM1）包含编码148个氨基酸的核苷酸、一个终止密码子，以及在A-A-T-A-A-A多聚腺苷酸添加信号之前的486个3'非编码序列残基。从这些核苷酸推导出来的氨基酸序列与牛脑钙调蛋白的氨基酸序列同源性为87%。cCM1是鸡基因组中两个钙调蛋白基因之一，但独特之处在于它不包含中断蛋白质结构片段的间隔序列。这表明cCM1起源于另一个钙调蛋白基因cCL1的加工基因拷贝，这种情况通常与假基因有关。相比之下，cCM1似乎是一个多基因家族的功能成员，其表达对肌肉细胞具有特异性。
