Sasaki K, Ichikawa M
Department of Anatomy, Yokohama City University, School of Medicine, Japan.
Tissue Cell. 1993;25(2):275-87. doi: 10.1016/0040-8166(93)90025-g.
The present study was designed to reveal membrane events during an erythrophagocytosis by electron microscopy. The erythrocytes, which were extravasated into rats' mesenteric lymph node sinuses by short clamping of the portal-vein and phagocytosed by fixed macrophages, were analysed at 0.5, 1, 3, 4 and 6 hr after reopening of the vein by using freeze-fracture and thin-section techniques. The results were as follows: the intramembranous particles of the phagocytosed erythrocyte aggregated after injection of lysosomal enzymes into the phagosome. The digested cytoplasm accumulated in the space between the phagosomal and erythrocyte membranes and was removed from the phagosome by bleb formation or invagination. Subsequently, the erythrocyte membranes lost their intramembranous particles and fragmented. The phagosome lost its density and erythrocyte-structure and became a vacuole (phagosomal ghost), which fractured lamellarly.
本研究旨在通过电子显微镜揭示红细胞吞噬过程中的膜事件。通过短暂夹闭门静脉使红细胞外渗至大鼠肠系膜淋巴结窦，并被固定的巨噬细胞吞噬，在静脉重新开放后0.5、1、3、4和6小时，采用冷冻断裂和超薄切片技术对这些红细胞进行分析。结果如下：向吞噬体注射溶酶体酶后，被吞噬红细胞的膜内颗粒聚集。消化后的细胞质积聚在吞噬体膜与红细胞膜之间的间隙中，并通过形成泡状或内陷从吞噬体中清除。随后，红细胞膜失去其膜内颗粒并破碎。吞噬体失去其密度和红细胞结构，变成一个液泡（吞噬体空壳），呈层状断裂。
