Elin R J, Tan H K
Blood. 1977 Apr;49(4):657-64.
This study investigated the anemia of dietary magnesium deficiency in inbred Fisher white rats using freeze-fracture electron microscopy. The plasma membranes of erythrocytes from animals receiving two different magnesium-deficient and control diets were observed at weekly or biweekly intervals for 6 wk. The earliest changes were small plaques on the external surface (ES) and fracture face (PF) of erythrocyte plasma membranes, which occurred after 2 wk of either magnesium-deficient diet. These plaques persisted and increased in size with progressive magnesium deficiency. When fully developed, the plaques consisted of round or oval elevations approximately 30-50 nm in diameter outlined by a narrow raised border. The surface of the plaques was smooth and devoid of intramembranous particles. Incubation of erythrocytes from magnesium-deficient rats in a physiologic solution containing 2 meq/liter magnesium for 1 hr at 37degrees C did not alter the appearance of the plaques. Erythrocytes from control rats, obtained during the same time periods, showed no plaques. Thus, a deficiency of magnesium in rats altered erythrocyte membrane structure.
本研究采用冷冻断裂电子显微镜技术，对近交系费希尔大白鼠因膳食镁缺乏所致的贫血进行了研究。以每周或每两周一次的间隔，持续6周观察接受两种不同缺镁饮食及对照饮食的动物红细胞的质膜。最早的变化是红细胞质膜外表面（ES）和断裂面（PF）上出现小斑块，这在两种缺镁饮食喂养2周后就会出现。随着镁缺乏程度的加重，这些斑块持续存在且尺寸增大。充分发展后，斑块由直径约30 - 50纳米的圆形或椭圆形隆起组成，周围有一条狭窄的凸起边界勾勒。斑块表面光滑，没有膜内颗粒。将缺镁大鼠的红细胞在含2毫当量/升镁的生理溶液中于37℃孵育1小时，并未改变斑块的外观。在同一时期获取的对照大鼠的红细胞未显示出斑块。因此，大鼠体内镁缺乏会改变红细胞膜结构。
