Demidova V S, Marchuk A I, Riapolova I V, Kozinets G I, Karelin A A
Vopr Med Khim. 1993 Mar-Apr;39(2):15-9.
Insulin-stimulated synthesis of plasma membraneous "signal" ATP (psATP) from ADP and P(i) in oxidation coupled with that of NADH was detected in a preparation of plasma membranes from human erythrocytes; psATP was formed at concentrations of 10(-8)-10(-9) M. Effect of medicinal plasmapheresis on ability of erythrocyte membranes to produce psATP was studied. The rate of psATP biosynthesis was estimated in healthy volunteers and in patients with various diseases: nonspecific aortic arteritis, bronchial asthma, peritonitis, myasthenia before and after plasmapheresis. Distinct values of basal content of ATP (without insulin) and insulin-stimulated biosynthesis of ATP were detected in volunteers. Elevation of ATP biosynthesis, in response to insulin effect, was equal to 8.029 +/- 0.163 nmol/mg of membrane protein per min. Estimation of the psATP biosynthesis rate in patients with various pathological states enabled to detect markedly that psATP content tends to increase after plasmapheresis. Absolute values of psATP content were distinctly lower in these patients than those in healthy volunteers, while after plasmapheresis these parameters approached the normal level. Estimation of the insulin-stimulated synthesis of psATP may serve as a valid criterion of plasmapheresis efficiency.
在人红细胞质膜制备物中检测到胰岛素刺激下由ADP和磷酸（Pi）合成质膜“信号”ATP（psATP），且该过程与NADH的氧化相偶联；psATP在10^(-8)-10^(-9) M的浓度下形成。研究了药物性血浆置换对红细胞膜产生psATP能力的影响。在健康志愿者以及患有各种疾病（非特异性主动脉动脉炎、支气管哮喘、腹膜炎、重症肌无力）的患者血浆置换前后，对psATP生物合成速率进行了评估。在志愿者中检测到了ATP（无胰岛素时）的基础含量以及胰岛素刺激下ATP的生物合成的不同值。响应胰岛素作用，ATP生物合成的升高量为每分钟8.029±0.163 nmol/mg膜蛋白。对各种病理状态患者的psATP生物合成速率进行评估后明显发现，血浆置换后psATP含量趋于增加。这些患者中psATP含量的绝对值明显低于健康志愿者，而血浆置换后这些参数接近正常水平。评估胰岛素刺激下psATP的合成可作为血浆置换效率的有效标准。
