Ge W, Gallin W J, Strobeck C, Peter R E
Department of Zoology, University of Alberta, Edmonton, Canada.
Biochem Biophys Res Commun. 1993 Jun 15;193(2):711-7. doi: 10.1006/bbrc.1993.1683.
Inhibin and activin are structurally related dimeric peptides sharing two distinct but related subunits, beta A and beta B. By using polymerase chain reaction (PCR), we have cloned and sequenced activin beta A and beta B genes encoding the mature region of the peptides from the goldfish genomic DNA. A single form of beta A and two forms of beta B subunits were found. There is high identity with mammalian counterparts; the goldfish beta A subunit has 78% protein sequence identity with human beta A subunit, and the two beta B subunits have more than 94% sequence identity with human beta B subunit. This is the first demonstration of the activin family of peptides in the fishes.
抑制素和激活素是结构相关的二聚体肽，共享两个不同但相关的亚基，βA和βB。通过使用聚合酶链反应（PCR），我们从金鱼基因组DNA中克隆并测序了编码这些肽成熟区域的激活素βA和βB基因。发现了一种形式的βA亚基和两种形式的βB亚基。与哺乳动物的对应物有高度同源性；金鱼βA亚基与人类βA亚基的蛋白质序列同源性为78%，两种βB亚基与人类βB亚基的序列同源性超过94%。这是首次在鱼类中证明激活素肽家族。
