Fang J, Yin W, Smiley E, Wang S Q, Bonadio J
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0417, USA.
Biochem Biophys Res Commun. 1996 Nov 21;228(3):669-74. doi: 10.1006/bbrc.1996.1715.
cDNA clones encoding a novel activin beta subunit have been isolated from mouse liver cDNA library. An amino acid homology comparison among members of the TGF-beta superfamily indicates that the open reading frame codes for a new activin/inhibin beta subunit. The predicted mature region of the subunit shows > 60% identity with activin beta C and beta D and > or = 45% identity with activin beta A and beta B, but only 20-40% amino acid sequence identity with other TGF-beta superfamily members. Therefore, the novel subunit has been designated activin beta E. Alignment of the mature growth factor region of the five activin beta subunits revealed that activin beta A and beta B share 63% amino acid identity, while activin beta C, beta D, and beta E share 62% identity. Therefore, activin beta A and beta B are most related to one another, while activin beta C, beta D, and beta E may represent a subset of related sequences.
编码一种新型激活素β亚基的cDNA克隆已从小鼠肝脏cDNA文库中分离出来。转化生长因子-β超家族成员之间的氨基酸同源性比较表明，开放阅读框编码一种新的激活素/抑制素β亚基。该亚基预测的成熟区域与激活素βC和βD具有>60%的同一性，与激活素βA和βB具有>或=45%的同一性，但与其他转化生长因子-β超家族成员的氨基酸序列同一性仅为20-40%。因此，该新型亚基被命名为激活素βE。五个激活素β亚基成熟生长因子区域的比对显示，激活素βA和βB的氨基酸同一性为63%，而激活素βC、βD和βE的同一性为62%。因此，激活素βA和βB彼此关系最为密切，而激活素βC、βD和βE可能代表相关序列的一个子集。
