Madewell B R, Grant C K
Am J Vet Res. 1977 Mar;38(3):333-5.
The 51Cr release assay was used to detect lysis of canine sarcoma cells incubated in vitro with alloantiserum and complement. Preincubation of the target cells with the anti-cancer agent doxorubicin-HCl increased the sensitivity of the cells to antibody-mediated lysis. The effect was dependent upon drug dose and was not reproduced by treatment with methotrexate, cytarabine, or dactinomycin. The observation suggests a possible ancillary mode of action for doxorubicin-HCl on neoplastic cells.
采用51Cr释放试验检测体外培养的犬肉瘤细胞与同种异体抗血清和补体孵育后的细胞裂解情况。用抗癌药物盐酸阿霉素对靶细胞进行预孵育可增加细胞对抗体介导裂解的敏感性。该效应取决于药物剂量，而甲氨蝶呤、阿糖胞苷或放线菌素D处理则不会产生同样的效果。这一观察结果提示盐酸阿霉素对肿瘤细胞可能存在一种辅助作用模式。
