针对犬瘟热的免疫机制。III. 补体溶解在犬瘟热病毒免疫和持续性感染中的作用。
Immune mechanisms against canine distemper. III. Role of complement lysis in the immunity and persistent infection of canine distemper virus.
作者信息
Ho C K, Babiuk L A
出版信息
Immunology. 1980 Feb;39(2):231-7.
Antibody-mediated complement lysis of Vero cells and canine macrophages infected with canine distemper virus (CDV) was demonstratee in an in vitro 51Cr release assay. This cytolytic activity was found to be highly efficient and was optimal under conditions which favoured the capping of redistribution of surface viral antigens. A prozone was observed in the presence of high antibody concentration and could not be eliminated by repeated washings. By tagging antibody-coated target cells with 125I-labelled staphylococcus protein A, it was found that the extent of protein A binding was parallel to the degree of cytotoxicity suggesting that the mechanism of this prozone effect was similar to that of a precipitation test.
在体外51Cr释放试验中证实了抗体介导的感染犬瘟热病毒（CDV）的Vero细胞和犬巨噬细胞的补体裂解作用。这种细胞溶解活性被发现是高效的……的英文原文似乎有误，正确的应该像是“Antibody-mediated complement lysis of Vero cells and canine macrophages infected with canine distemper virus (CDV) was demonstrated in an in vitro 51Cr release assay. This cytolytic activity was found to be highly efficient and was optimal under conditions which favored the capping induced by redistribution of surface viral antigens. A prozone was observed in the presence of high antibody concentration and could not be eliminated by repeated washings. By tagging antibody-coated target cells with 125I-labelled staphylococcus protein A, it was found that the extent of protein A binding was parallel to the degree of cytotoxicity suggesting that the mechanism of this prozone effect was similar to that of a precipitation test.”
试译如下
在体外51Cr释放试验中证实了抗体介导的感染犬瘟热病毒（CDV）的Vero细胞和犬巨噬细胞的补体裂解作用。发现这种细胞溶解活性非常高效，并且在有利于由表面病毒抗原重新分布诱导的帽化作用的条件下最为理想。在高抗体浓度存在的情况下观察到前带现象，并且通过反复洗涤无法消除。通过用125I标记的葡萄球菌蛋白A标记抗体包被的靶细胞，发现蛋白A结合程度与细胞毒性程度平行，这表明这种前带效应的机制与沉淀试验的机制相似。
Zotero 插件