一种纯化寡脱氧核糖核苷甲基膦酸酯的简单快速方法。
A simple and rapid method for purification of oligodeoxyribonucleoside methylphosphonates.
作者信息
Lin S B, Chang G W, Teh G W, Lin K I, Au L C
机构信息
Department of Clinical Pathology, College of Medicine, National Taiwan University, Republic of China.
出版信息
Biotechniques. 1993 May;14(5):795-8.
PMID:8512705
Abstract
An alternative dimethoxytrityl-on (dmt-on) method is described to purify hydrophobic oligodeoxyribonucleoside methyl-phosphonates (OM) with a phosphodiester linkage at the 5' end, instead of the conventional dmt-off method using a DEAE ion-exchange column. This method is modified from the reverse-phase method for purification of normal oligonucleotides.
摘要
本文描述了一种替代的二甲氧基三苯甲基在位（DMT在位）方法，用于纯化5'端带有磷酸二酯键的疏水寡脱氧核糖核苷甲基膦酸酯（OM），而不是使用DEAE离子交换柱的传统DMT脱除方法。该方法是从用于纯化普通寡核苷酸的反相方法改进而来的。
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