针对兔脑炎微孢子虫的直接ABC免疫组织化学法。

Direct ABC immunohistochemistry to Encephalitozoon cuniculi.

作者信息

Park J H, Ochiai K, Itakura C

机构信息

Department of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

出版信息

J Vet Med Sci. 1993 Apr;55(2):325-8. doi: 10.1292/jvms.55.325.

DOI:10.1292/jvms.55.325

PMID:8513018

Abstract

Among the sera from 9 rabbits spontaneously infected with Encephalitozoon cuniculi, a serum which revealed a high titer for E. cuniculi by indirect protein A-gold (IPAG) immunohistochemistry and reacted with the outer layer of the shell of E. cuniculi spores on immunoelectron microscopical examination, was biotinylated. The biotinylated rabbit anti-E. cuniculi IgG reacted immunohistochemically with E. cuniculi, but not with other protozoa tested, namely Neospora caninum, Toxoplasma gondii and sarcocystis. The direct avidin-biotin-peroxidase complex (ABC) immunohistochemistry using biotinylated rabbit anti-E. cuniculi IgG in this study is a useful tool for the diagnosis and study of encephalitozoonosis.

摘要

在9只自然感染兔脑炎微孢子虫的兔子血清中，选取一份通过间接蛋白A-金（IPAG）免疫组化显示对兔脑炎微孢子虫具有高滴度且在免疫电镜检查中与兔脑炎微孢子虫孢子外壳外层发生反应的血清进行生物素化处理。生物素化的兔抗兔脑炎微孢子虫IgG通过免疫组化与兔脑炎微孢子虫发生反应，但与其他受试原生动物，即犬新孢子虫、刚地弓形虫和肉孢子虫不发生反应。本研究中使用生物素化兔抗兔脑炎微孢子虫IgG的直接抗生物素蛋白-生物素-过氧化物酶复合物（ABC）免疫组化是诊断和研究兔脑炎微孢子虫病的有用工具。