Hurley W L, Rejman J J
Department of Animal Sciences, University of Illinois, Urbana 61801.
Cell Biol Int. 1993 Mar;17(3):283-9. doi: 10.1006/cbir.1993.1064.
Bovine lactoferrin in involuting mammary tissue was identified by immunohistochemistry and tissue explant culture. Immunoreactive lactoferrin was associated with mammary epithelial cells. Immunostaining for lactoferrin increased during involution, in contrast to declining immunostaining of epithelia for the milk-specific protein beta-lactoglobulin. Immunostaining for lactoferrin also was observed at the basal region of alveolar epithelia, perhaps in association with basement membrane components. Lactoferrin was preferentially synthesized in involuting mammary tissue compared with lactating tissue. Synthesis of lactoferrin in the involuting mammary gland occurs despite the apparent decline in synthesis of milk-specific proteins.
通过免疫组织化学和组织外植体培养鉴定了退化乳腺组织中的牛乳铁蛋白。免疫反应性乳铁蛋白与乳腺上皮细胞相关。与乳腺上皮细胞中乳特异性蛋白β-乳球蛋白免疫染色下降相反，乳铁蛋白的免疫染色在退化过程中增加。在肺泡上皮细胞的基底区域也观察到乳铁蛋白的免疫染色，可能与基底膜成分有关。与泌乳组织相比，乳铁蛋白在退化乳腺组织中优先合成。尽管乳特异性蛋白的合成明显下降，但退化乳腺中仍会发生乳铁蛋白的合成。
