Hurst R E, Bynum R L, Rhodes S W
Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Cancer Lett. 1993 May 14;69(3):197-202. doi: 10.1016/0304-3835(93)90175-9.
Heparin at concentrations below 100 micrograms/ml stimulated anchorage-independent growth of NRK 49F (normal rat kidney fibroblasts, American type culture collection) rat fibroblasts at suboptimal cytokine concentrations but inhibited it at higher heparin concentrations regardless of the cytokine concentration. Heparin did not stimulate growth above that seen at optimal cytokine concentrations, suggesting that it alters the cellular response to the cytokines. These data suggest natural protein-glycosaminoglycan interactions play a role in modulating or mediating the actions of transforming cytokines and suggest they may play a role in acquisition of the transformed phenotype.
浓度低于100微克/毫升的肝素在亚最佳细胞因子浓度下刺激NRK 49F（正常大鼠肾成纤维细胞，美国典型培养物保藏中心）大鼠成纤维细胞的非锚定依赖性生长，但在较高肝素浓度下抑制其生长，而与细胞因子浓度无关。肝素不会刺激超过最佳细胞因子浓度时所观察到的生长，这表明它改变了细胞对细胞因子的反应。这些数据表明天然蛋白质-糖胺聚糖相互作用在调节或介导转化细胞因子的作用中发挥作用，并表明它们可能在转化表型的获得中发挥作用。
