新生大鼠的短间隔杏仁核点燃
Short-interval amygdala kindling in neonatal rats.
作者信息
Baram T Z, Hirsch E, Schultz L
机构信息
Department of Neurology, University of Southern California, Childrens Hospital Los Angeles 90054-0700.
出版信息
Brain Res Dev Brain Res. 1993 May 21;73(1):79-83. doi: 10.1016/0165-3806(93)90048-f.
The kindling paradigm provides a powerful tool for studying the generation, propagation and generalization of seizures. Such reproducible quantitative paradigms are a prerequisite for the experimental study of epilepsy in the developing brain. Kindling has been extensively utilized as a model of limbic seizures in the adult rat; amygdala short-interval kindling has been studied in > or = 15-day-old rats. We applied the short-interval kindling method, i.e., stimulation at every 15 min, to 7-12-day-old rats. Stage-5 behavioral seizures were achieved even in 7-day-old rats; however, the progression of behavioral kindling differed somewhat from that of older rats. Correlation of electrographic discharges and behavioral phenomena was inversely related to age. Reliable progressive amygdala discharges were difficult to assess in most < or = 10-day-old rats. Spontaneous seizures occurred relatively frequently in younger age groups. The amygdala short-interval kindling paradigm is reproducibly and reliably applicable to rats during the 2nd postnatal week. The presence of progressive focal to bilateral-generalized seizures suggests a significant functional maturity of the amygdala-limbic circuitry at this age.
点燃模型为研究癫痫发作的产生、传播和泛化提供了一个强大的工具。这种可重复的定量模型是发育中大脑癫痫实验研究的先决条件。点燃已被广泛用作成年大鼠边缘性癫痫发作的模型；杏仁核短间隔点燃已在≥15日龄的大鼠中进行了研究。我们将短间隔点燃方法，即每15分钟刺激一次，应用于7-12日龄的大鼠。即使在7日龄的大鼠中也能达到5级行为性癫痫发作；然而，行为性点燃的进展与年龄较大的大鼠有所不同。脑电图放电与行为现象的相关性与年龄呈负相关。在大多数≤10日龄的大鼠中，难以评估可靠的进行性杏仁核放电。在较年轻的年龄组中，自发性癫痫发作相对频繁。杏仁核短间隔点燃模型在出生后第二周可重复且可靠地应用于大鼠。进行性局灶性至双侧全身性癫痫发作的出现表明，在这个年龄，杏仁核-边缘回路具有显著的功能成熟度。
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