Simon A, Palanker D, Harpaz-Eisenberg V, Lewis A, Laufer N
Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel.
Fertil Steril. 1993 Jul;60(1):159-64. doi: 10.1016/s0015-0282(16)56055-5.
To provide conclusive evidence that sperm cells gain access to the perivitelline space exclusively through a laser-drilled opening. To assess the optimal size of the hole and to evaluate the efficacy of laser drilling in comparison with that of mechanical zona dissection.
An interspecies model of human sperm cell that interacts with a laser-drilled or partially zona-dissected hamster oocytes.
Penetration rate into the perivitelline space as related to the size of the opening (group A [5 microns], group B [10 microns], and group C [15 microns]) and to the sperm cell concentrations (1 x 10(6), 5 x 10(6), and 10 x 10(6) cells/mL) used for insemination.
For each sperm cell concentration, the penetration rate into the perivitelline space was lowest for group A followed by group C and highest for group B. When penetration was compared for each hole size, it was found that sperm concentration had no effect on the rate of penetration in groups A and C but significantly affected this rate in group B. The highest penetration rate of 73% was observed with a concentration of 10 x 10(6) cell/mL and declined to 58% and 23% at 5 x 10(6) cell/mL and 1 x 10(6) cell/mL, respectively. Oocytes drilled by laser (10-microns hole) demonstrated a significantly higher penetration rate when compared with those treated by partial zona dissection (73% versus 20% and 58% versus 21% for sperm densities of 10 x 10(6) cells/mL and 5 x 10(6) cells/mL, respectively).
Human sperm cells gain access into the perivitelline space of hamster oocytes exclusively through a hole drilled by an argon fluoride excimer laser. An opening of 10 microns was found to yield optimal results. Laser drilling of the zona pellucida seems to be superior to that of mechanical slitting in terms of sperm oolema interaction.
提供确凿证据，证明精子细胞仅通过激光打孔进入卵周隙。评估孔的最佳大小，并与机械性透明带切开术相比，评估激光打孔的效果。
人类精子细胞与激光打孔或部分透明带切开的仓鼠卵母细胞相互作用的种间模型。
与开口大小（A组[5微米]、B组[10微米]和C组[15微米]）以及用于授精的精子细胞浓度（1×10⁶、5×10⁶和10×10⁶个细胞/毫升）相关的进入卵周隙的穿透率。
对于每种精子细胞浓度，A组进入卵周隙的穿透率最低，其次是C组，B组最高。当比较每个孔大小的穿透情况时，发现精子浓度对A组和C组的穿透率没有影响，但对B组的穿透率有显著影响。在浓度为10×10⁶个细胞/毫升时观察到最高穿透率为73%，在5×10⁶个细胞/毫升和1×10⁶个细胞/毫升时分别降至58%和23%。与部分透明带切开处理的卵母细胞相比，激光打孔（10微米孔）的卵母细胞显示出显著更高的穿透率（精子密度为10×10⁶个细胞/毫升时分别为73%对20%，5×10⁶个细胞/毫升时为58%对21%）。
人类精子细胞仅通过氟化氩准分子激光打孔进入仓鼠卵母细胞的卵周隙。发现10微米的开口可产生最佳结果。就精子与卵细胞膜的相互作用而言，透明带的激光打孔似乎优于机械切开。
