Reid B J, Sanchez C A, Blount P L, Levine D S
Department of Medicine, University of Washington, Seattle.
Gastroenterology. 1993 Jul;105(1):119-29. doi: 10.1016/0016-5085(93)90017-7.
Abnormal proliferation in Barrett's esophagus may predispose to the development of esophageal adenocarcinoma, but previous studies have not determined the specific cell cycle abnormalities that were associated with neoplastic progression.
Ki67/DNA content multiparameter flow cytometry and DNA content flow cytometry were used to investigate G0, G1, and S phase fractions in advancing stages of neoplastic progression in Barrett's esophagus.
In control biopsy specimens from gastric mucosa, G1, S phase, and total Ki67-positive proliferative fractions were low, suggesting that cells were predominantly in G0. Ki67-positive G1 fractions were increased in Barrett's metaplasia. More advanced stages of neoplastic progression were characterized by a subset of biopsy specimens that had aneuploid cell populations, increased S phase fractions, or both.
The development of increased G1 fractions is an early event in Barrett's metaplasia. Increased S phase fractions occur in a subset of specimens typically at more advanced stages of neoplastic progression and often in association with the development of aneuploidy. Neoplastic progression in Barrett's esophagus is associated with at least three types of cell cycle abnormalities: (1) mobilization from G0 into G1; (2) loss of control of the G1/S phase transition; and (3) accumulation in G2.
巴雷特食管中的异常增殖可能易导致食管腺癌的发生，但以往研究尚未确定与肿瘤进展相关的具体细胞周期异常情况。
采用Ki67/DNA含量多参数流式细胞术和DNA含量流式细胞术，研究巴雷特食管肿瘤进展各阶段的G0、G1和S期比例。
在胃黏膜对照活检标本中，G1期、S期以及总的Ki67阳性增殖比例较低，提示细胞主要处于G0期。巴雷特化生中Ki67阳性G1期比例增加。肿瘤进展更高级阶段的特征是部分活检标本具有非整倍体细胞群、S期比例增加或两者皆有。
G1期比例增加是巴雷特化生的早期事件。S期比例增加出现在部分标本中，通常是在肿瘤进展的更高级阶段，且常与非整倍体的发生相关。巴雷特食管的肿瘤进展与至少三种细胞周期异常有关：（1）从G0期进入G1期；（2）G1/S期转换失控；（3）在G2期积累。
