Jones K H, Smith R T, Trudgill P W
Department of Biochemistry, University of Wales, Aberystwyth, UK.
J Gen Microbiol. 1993 Apr;139(4):797-805. doi: 10.1099/00221287-139-4-797.
Pseudomonas putida ATCC 17453 grew with either (+)- or (-)-camphor as sole carbon source. Enantiomer-specific 'biological Baeyer-Villiger' monooxygenases were synthesized irrespective of the camphor isomer used for growth. The two enzymes are probably the products of separate genes but showed many similarities. Each consisted of two electrophoretically identical subunits, bound flavin mononucleotide (FMN) non-covalently and accepted electrons from an induced NADH dehydrogenase which interacted with the FMN bound to the oxygenating component. They showed minor differences in M(r) with 3,6-diketocamphane 1,6-monooxygenase being the smaller enzyme. Isoelectric focussing showed the two enzymes to have different acidic pI values. Polyclonal antibodies raised against 3,6-diketocamphane 1,6-monooxygenase also cross-reacted with 2,5-diketocamphane 1,2-monooxygenase and its subunits.
恶臭假单胞菌ATCC 17453能够以（+）-或（-）-樟脑作为唯一碳源生长。无论用于生长的樟脑异构体是哪种，都会合成对映体特异性的“生物拜耳-维利格”单加氧酶。这两种酶可能是不同基因的产物，但表现出许多相似之处。每种酶都由两个电泳性质相同的亚基组成，非共价结合黄素单核苷酸（FMN），并从一种诱导型NADH脱氢酶接受电子，该脱氢酶与结合在氧化成分上的FMN相互作用。它们在相对分子质量上有微小差异，3,6-二酮樟脑1,6-单加氧酶是较小的酶。等电聚焦显示这两种酶具有不同的酸性等电点值。针对3,6-二酮樟脑1,6-单加氧酶产生的多克隆抗体也与2,5-二酮樟脑1,2-单加氧酶及其亚基发生交叉反应。
