Tinoco J, Miljanich P, Medwadowski B
Biochim Biophys Acta. 1977 Mar 25;486(3):575-8. doi: 10.1016/0005-2760(77)90111-4.
Rats were raised for 2 generations on a diet in which 1.25% methyl linoleate was the only source of fat. Control rats were given 1.0% methyl linoleate plus 0.25% methyl linolenate. Lipids were extracted from retinas and their fatty acids were analyzed by gas-liquid chromatography. Docosahexaenoic acid accounted for 33.8% of total fatty acids in control retinas, for 13% of fatty acids in first-generation deficient retinas, and for 2.7% of fatty acids in second-generation deficient retinas.
大鼠在以1.25%亚油酸甲酯作为唯一脂肪来源的饮食条件下饲养两代。对照大鼠给予1.0%亚油酸甲酯加0.25%亚麻酸甲酯。从视网膜中提取脂质，并通过气液色谱法分析其脂肪酸。二十二碳六烯酸在对照视网膜的总脂肪酸中占33.8%，在第一代缺乏该脂肪酸的视网膜中占脂肪酸的13%，在第二代缺乏该脂肪酸的视网膜中占脂肪酸的2.7%。
