Dvorak A M, Seder R A, Paul W E, Kissell-Rainville S, Plaut M, Galli S J
Department of Pathology, Beth Israel Hospital, Boston, Massachusetts.
Lab Invest. 1993 Jun;68(6):708-15.
Previous work showed that injection of mice with goat anti-mouse IgD antibodies results in increased numbers of Fc epsilon R-positive, non-B, non-T cells in the spleen and Fc epsilon R-positive cells in the bone marrow, and that some of these cells had ultrastructural features of basophils. Fc epsilon R-positive, non-B, non-T cells express virtually all of the capacity of mouse splenic "non-B, non-T cells" to produce interleukin-4 in response to stimulation by cross-linking of Fc epsilon R or Fc gamma R, or by the calcium ionophore, ionomycin.
The present study is a detailed ultrastructural analysis of Fc epsilon R-positive bone marrow cells or Fc epsilon R-positive splenic non-B, non-T cells sorted from mice injected with goat anti-mouse IgD antibody and of Fc epsilon R-positive bone marrow cells or spleen cells pooled from normal mice not injected with goat anti-IgD.
Basophils represented the majority (90%) of the granulated cells present in the Fc epsilon R-positive splenic non-B, non-T cells or Fc epsilon R-positive bone marrow cells of goat anti-IgD-injected mice. In contrast, the cytoplasmic granule-containing Fc epsilon R-negative cells sorted from spleen or bone marrow of goat anti-IgD-injected animals contained predominantly a mixture of neutrophils, eosinophils, monocytes and their precursors. Both the Fc epsilon R-positive and -negative preparations contained rare (< 5%) cells with ultrastructural features of very immature mast cells. Basophils were also identified in Fc epsilon R-positive cells sorted from total bone marrow cells or spleen cells of normal mice not injected with goat anti-IgD.
Taken together with data concerning the numbers of Fc epsilon R-positive, non-B, non-T cells in the spleen, and Fc epsilon R-positive B220-negative cells in the bone marrow, these ultrastructural findings indicate that injection of mice with goat anti-IgD results in increased numbers of basophils, particularly in the spleen, that exhibit an 8-fold increase in basophils as a result of injection of goat anti-IgD.
先前的研究表明，给小鼠注射山羊抗小鼠IgD抗体后，脾脏中FcεR阳性、非B、非T细胞以及骨髓中FcεR阳性细胞的数量会增加，并且其中一些细胞具有嗜碱性粒细胞的超微结构特征。FcεR阳性、非B、非T细胞几乎表达了小鼠脾脏“非B、非T细胞”在FcεR或FcγR交联刺激、或钙离子载体离子霉素刺激下产生白细胞介素-4的全部能力。
本研究对从注射了山羊抗小鼠IgD抗体的小鼠中分离出的FcεR阳性骨髓细胞或FcεR阳性脾脏非B、非T细胞，以及从未注射山羊抗IgD的正常小鼠中收集的FcεR阳性骨髓细胞或脾细胞进行了详细的超微结构分析。
在注射了山羊抗IgD的小鼠的FcεR阳性脾脏非B、非T细胞或FcεR阳性骨髓细胞中，嗜碱性粒细胞占颗粒细胞的大多数（90%）。相比之下，从注射了山羊抗IgD的动物的脾脏或骨髓中分离出的含细胞质颗粒的FcεR阴性细胞主要包含中性粒细胞、嗜酸性粒细胞、单核细胞及其前体的混合物。FcεR阳性和阴性制剂中均含有罕见（<5%）的具有非常不成熟肥大细胞超微结构特征的细胞。在从未注射山羊抗IgD的正常小鼠的全骨髓细胞或脾细胞中分离出的FcεR阳性细胞中也鉴定出了嗜碱性粒细胞。
结合有关脾脏中FcεR阳性、非B、非T细胞数量以及骨髓中FcεR阳性B220阴性细胞数量的数据，这些超微结构发现表明，给小鼠注射山羊抗IgD会导致嗜碱性粒细胞数量增加，尤其是在脾脏中，注射山羊抗IgD后嗜碱性粒细胞数量增加了8倍。
