[使用定向扩增技术诊断支原体感染]

[Diagnosis of mycoplasma infections using directed amplification].

作者信息

Zhulanova E Iu, Vonskiĭ M S, Lisin V V, Efremova T N, Borkhsenius S N

出版信息

Mol Gen Mikrobiol Virusol. 1993 Mar-Apr(2):9-13.

Abstract

Test systems for rapid detection of mycoplasmas in biological samples have been elaborated on the base of the polymerase chain reaction (PCR). Amplification of the conservative rDNA sequences was used for testing of cell cultures for mycoplasmal contamination. Mycoplasma pneumoniae detection system has been developed based on amplification of the species-specific DNA sequences. Inversions of some repeated sequences in the Mycoplasma pneumoniae genome make it possible to run the PCR with a single primer. The revealed spacer length polymorphism for 16S-23S rDNA operons can be used in the mycoplasmas identification.

摘要

基于聚合酶链反应（PCR），已研发出用于快速检测生物样本中支原体的检测系统。保守的rDNA序列扩增用于检测细胞培养物是否受到支原体污染。基于物种特异性DNA序列的扩增，开发了肺炎支原体检测系统。肺炎支原体基因组中一些重复序列的倒置使得使用单一引物进行PCR成为可能。所揭示的16S - 23S rDNA操纵子的间隔区长度多态性可用于支原体鉴定。