Perlman R, Yablonski D, Simchen G, Levitzki A
Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel.
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5474-8. doi: 10.1073/pnas.90.12.5474.
The STE5 gene of Saccharomyces cerevisiae was cloned using a screening procedure designed to isolate genes of the S. cerevisiae pheromone response pathway. We screened a yeast genomic high-copy-number plasmid library for genes that allow mating of cdc25ts mutants at the restrictive temperature without affecting the cell-cycle-arrest phenotype. One of the genes cloned was identified by genetic analysis as STE5. STE5 encodes a predicted open reading frame of 916 amino acids and exhibits significant homology to Far1 protein. RNA blot analysis reveals that STE5 gene transcription is regulated by the mating type of the cell and depends on an intact pheromone-response pathway.
利用一种旨在分离酿酒酵母信息素反应途径基因的筛选程序，克隆了酿酒酵母的STE5基因。我们在酵母基因组高拷贝数质粒文库中筛选那些能使cdc25ts突变体在限制温度下进行交配而不影响细胞周期阻滞表型的基因。克隆到的其中一个基因经遗传分析鉴定为STE5。STE5编码一个由916个氨基酸组成的预测开放阅读框，并且与Far1蛋白具有显著的同源性。RNA印迹分析表明，STE5基因的转录受细胞交配型的调控，并且依赖于完整的信息素反应途径。
