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[根据电子显微镜放射自显影结果对小鼠大脑皮质细胞中RNA合成的研究]

[RNA synthesis in cells of the cerebral cortex of mice according to electron-microscopic autoradiography findings].

作者信息

Pal'tsyn A A, Sanovich E Ia, Tumanov V P

出版信息

Biull Eksp Biol Med. 1977 Mar;83(3):364-6.

PMID:851640
Abstract

An electron microscopy autoratiographic study of the RNA synthesis in the neurons of the mouse cerebral cortex revealed the highest label concentration in the nucleoli. A considerable amount of silver grains was concentrated over the nucleoplasm. The content of the radioactive substance in the cytoplasm of the neurons 2 1/2 hours after the animal was injected with 3H-5-uridine was lower than in the other kinds of cells at the same period of their contact with the labeled precursor. A considerable difference in the amount of silver grains was observed over the serial sections of the same nucleolus and in the way of the label distribution in the neurons located nearby.

摘要

一项关于小鼠大脑皮质神经元中RNA合成的电子显微镜放射自显影研究显示,核仁中的标记浓度最高。相当数量的银颗粒集中在核质上。给动物注射3H-5-尿苷2.5小时后,神经元细胞质中的放射性物质含量低于同一时期与标记前体接触的其他种类细胞。在同一个核仁的连续切片上以及在附近神经元中标记分布的方式上,观察到银颗粒数量存在显著差异。

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