Wenz B, Ciavarella D, Freundlich L
Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, New York.
Transfusion. 1993 Jun;33(6):520-3. doi: 10.1046/j.1537-2995.1993.33693296817.x.
Platelet concentrates stored with and without autologous white cells were produced from units of whole blood that had been purposefully contaminated with bacteria immediately after phlebotomy. The blood was inoculated with one of five species of bacterium at either 10 or 50 colony-forming units per mL. The growth of the organisms was quantified throughout the conventional 5-day, 22 degrees C storage period of the platelet concentrates. One species, Klebsiella pneumoniae, failed to grow in any of the components. The remaining species, Staphylococcus epidermidis, S. aureus, Enterococcus faecalis, and Salmonella enteritidis, achieved log growth after 1 day of storage and reached a relative maximum concentration by Day 3. Although the concentration of bacteria immediately after inoculation was lower in the units reduced in white cells by filtration, no significant differences were observed thereafter. Data from this in vitro study support the concept that prestorage white cell reduction of platelet concentrates should not increase the likelihood of transfusion-induced septicemia.
将刚采血后故意接种细菌的全血样本制备成含有和不含自体白细胞的血小板浓缩物。血液接种了五种细菌中的一种，接种浓度为每毫升10或50个菌落形成单位。在血小板浓缩物常规的5天、22℃储存期内对微生物的生长进行定量分析。有一个菌种，即肺炎克雷伯菌，在任何成分中均未生长。其余菌种，即表皮葡萄球菌、金黄色葡萄球菌、粪肠球菌和肠炎沙门氏菌在储存1天后实现对数生长，并在第3天达到相对最高浓度。尽管通过过滤减少白细胞的样本接种后立即检测到的细菌浓度较低，但此后未观察到显著差异。这项体外研究的数据支持这样一个概念，即血小板浓缩物储存前减少白细胞不应增加输血后败血症的可能性。
