Katakura K, Matsumoto Y, Gomez E A, Furuya M, Hashiguchi Y
Department of Parasitology, Jikei University School of Medicine, Tokyo, Japan.
Am J Trop Med Hyg. 1993 May;48(5):707-15. doi: 10.4269/ajtmh.1993.48.707.
Molecular karyotypes of Leishmania isolates from patients with cutaneous leishmaniasis in Ecuador were analyzed by pulsed-field gel electrophoresis (PFGE) and Southern blot hybridization. The DNA karyotypes of L. major-like parasites were similar between two human isolates from a lowland coastal and a highland Andean region, but were apparently different from those of eleven World Health Organization reference strains including L. major. The smallest chromosome of 240 kilobases in L. major-like parasites was found to belong to the 715-class of small linear chromosomal DNAs, which have been shown to appear in some lines of Leishmania. Chromosome banding patterns of L. mexicana isolates exhibited a novel, ordered, chromosomal ladder, and were identical among four human isolates and one canine isolate from a restricted geographic region in the Andes. On the other hand, minor chromosome size polymorphisms were observed among three L. panamensis isolates from different endemic regions near the Pacific Coast. Chromosomal locations of dihydrofolate reductase-thymidylate synthetase and P-glycoprotein genes revealed further differences in chromosomal organizations among these Leishmania species in Ecuador. These results indicate that karyotype analysis by PFGE is useful for epidemiologic studies of leishmaniasis in Ecuador.
通过脉冲场凝胶电泳（PFGE）和Southern印迹杂交分析了来自厄瓜多尔皮肤利什曼病患者的利什曼原虫分离株的分子核型。来自低地沿海和安第斯高地地区的两个人类分离株中，类硕大利什曼原虫的DNA核型相似，但明显不同于包括硕大利什曼原虫在内的11种世界卫生组织参考菌株的核型。在类硕大利什曼原虫中，发现最小的240千碱基染色体属于715类小线性染色体DNA，已证明其出现在某些利什曼原虫系中。墨西哥利什曼原虫分离株的染色体带型呈现出一种新颖、有序的染色体梯状图谱，并且在来自安第斯山脉一个有限地理区域的四个人类分离株和一个犬类分离株中是相同的。另一方面，在来自太平洋沿岸附近不同流行地区的三个巴拿马利什曼原虫分离株中观察到了较小的染色体大小多态性。二氢叶酸还原酶-胸苷酸合成酶和P-糖蛋白基因的染色体定位揭示了厄瓜多尔这些利什曼原虫物种在染色体组织上的进一步差异。这些结果表明，PFGE核型分析对厄瓜多尔利什曼病的流行病学研究有用。
