Hernández F J, Goyache J, Orden J A, Blanco J L, Doménech A, Suárez G, Gómez-Lucía E
Departmento Patología Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Appl Environ Microbiol. 1993 May;59(5):1515-9. doi: 10.1128/aem.59.5.1515-1519.1993.
To study repair and enterotoxin synthesis, four staphylococcal strains (FRI-100, FRI-137, FRI-472, and S6) were subjected to sublethal heat treatment, transferred to four liquid repair media (1% powdered skim milk in distilled water, complex medium, M9 minimal salt medium, and saline solution), and then incubated at different temperatures. Powdered skim milk proved to be the most efficient medium for promoting the repair of injured cells, particularly at 37 degrees C. Minimal salt medium also gave good results. Salt tolerance also increased at 4 degrees C, although it did not reach normal values. After 6 h of incubation at 37 degrees C in powdered skim milk, strain FRI-100 synthesized detectable amounts of enterotoxin A. After 10 h of incubation in the same medium at the same temperature, enterotoxins were detected in all of the strains.
为了研究修复和肠毒素合成情况，对四株葡萄球菌菌株（FRI - 100、FRI - 137、FRI - 472和S6）进行亚致死热处理，将其转移至四种液体修复培养基（蒸馏水中含1%脱脂奶粉、复合培养基、M9基本盐培养基和盐溶液）中，然后在不同温度下培养。结果表明，脱脂奶粉是促进受损细胞修复最有效的培养基，尤其是在37℃时。基本盐培养基也取得了良好效果。在4℃时耐盐性也有所提高，尽管未达到正常值。在脱脂奶粉中于37℃培养6小时后，FRI - 100菌株合成了可检测量的肠毒素A。在相同温度下于该培养基中培养10小时后，在所有菌株中均检测到了肠毒素。
