Martin S E, Chaven S
Appl Environ Microbiol. 1987 Jun;53(6):1207-9. doi: 10.1128/aem.53.6.1207-1209.1987.
During the growth of Staphylococcus aureus MF-31, initial catalase activity dropped to a reduced level at the onset of exponential phase before increasing. When S. aureus was grown at 25, 32, or 37 degrees C, catalase activity was found to decrease by 80 to 90% within 1 h of inoculation. Two catalase-negative mutants and wild-type S. aureus MF-31 cells were exposed to exogenous 20 mM H2O2 for 15 min. For wild-type S. aureus, there was no effect from H2O2 until min 15, at which time a 10% decrease in CFU was observed. Both mutants showed increased sensitivity to the H2O2, with 56 and 71% reductions in the CFU for mutants C3 and C4, respectively, after a 15-min exposure. Cells of mutant and wild-type S. aureus were subjected to sublethal heating at 52 degrees C for 20 min. The lack of catalase activity in the mutants resulted in large decreases in enumeration.
在金黄色葡萄球菌MF-31生长期间，初始过氧化氢酶活性在指数期开始时降至较低水平，随后才升高。当金黄色葡萄球菌在25、32或37摄氏度下生长时，发现接种后1小时内过氧化氢酶活性降低80%至90%。将两个过氧化氢酶阴性突变体和野生型金黄色葡萄球菌MF-31细胞暴露于20 mM外源过氧化氢中15分钟。对于野生型金黄色葡萄球菌，直到第15分钟过氧化氢才产生影响，此时观察到菌落形成单位（CFU）下降了10%。两个突变体对过氧化氢的敏感性均增加，暴露15分钟后，突变体C3和C4的CFU分别减少了56%和71%。将突变型和野生型金黄色葡萄球菌细胞在52摄氏度下进行亚致死加热20分钟。突变体中缺乏过氧化氢酶活性导致计数大幅下降。
