Krawielitzki K, Schadereit R, Kreienbring F
Institut für Biologie der landwirtschaftlichen Nutztiere, Forschungsbereich Ernährungsphysiologie Oskar Kellner Rostock, Germany.
Arch Tierernahr. 1993;43(4):303-12. doi: 10.1080/17450399309386045.
The fractional synthesis rates (FSR) for liver, pancreas, small intestine, large intestine, skeleton muscle and whole body protein were estimated with the help of the large dose method of McNurlan et al. (1979) and Garlick et.al (1980) using simultaneously [14C]lysine and [15N]lysine as flooding substances in growing rats. The following results and conclusions can be drawn: In the case of lysine as flooding substance the incorporation time (time from the injection up to killing the animals) can be fixed at 20 minutes. On principle lysine is suitable as flooding substance. [14C]lysine and [15N]lysine can yield identical values for FSR in the same animal. The variation coefficients of the FSR values were 7.3% (4... 12%) using [14C]lysine and 13.8% (9.5... 36%) using [15N]lysine (emission-spectrometry) as flooding substance. Using the mass-spectrometric method for measuring 15N-excess it is possible to obtain the same accuracy of result as in the case of estimation [14C]lysin. The main advantage of using 15N-labelled amino acids as flooding substances is the absence of any radioactivity. Therefore this method is also suitable for farm animals and--in combination with the indolent muscle biopsy--it may be used for men.
采用McNurlan等人（1979年）和Garlick等人（1980年）的大剂量法，以[14C]赖氨酸和[15N]赖氨酸作为灌流剂，同时测定生长大鼠肝脏、胰腺、小肠、大肠、骨骼肌和全身蛋白质的分数合成率（FSR）。可得出以下结果和结论：以赖氨酸作为灌流剂时，掺入时间（从注射到处死动物的时间）可确定为20分钟。原则上，赖氨酸适合作为灌流剂。在同一动物中，[14C]赖氨酸和[15N]赖氨酸可得出相同的FSR值。以[14C]赖氨酸作为灌流剂时，FSR值的变异系数为7.3%（4...12%），以[15N]赖氨酸（发射光谱法）作为灌流剂时，变异系数为13.8%（9.5...36%）。采用质谱法测量15N过量时，可获得与估算[14C]赖氨酸时相同的结果准确性。使用15N标记氨基酸作为灌流剂的主要优点是不存在任何放射性。因此，该方法也适用于家畜，并且——与惰性肌肉活检相结合——可用于人类。
