Butter J J, Kraak J C, Poppe H
Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
J Pharm Biomed Anal. 1993 Mar;11(3):225-32. doi: 10.1016/0731-7085(93)80201-b.
An on-line coupled HPLC system is described for the determination of the enantiomers of bupivacaine in serum. The method involves three steps: (i) pre-concentration and clean-up; (ii) the determination of the racemates on a reversed-phase column; and (iii) the separation of the racemates, heart-cut from the reversed-phase column, on an alpha-glycoprotein column. The method is suitable to determine the enantiomers in serum down to 0.1 micrograms ml-1 and can be fully automated. The bupivacaine time course of patients will be shown.
描述了一种用于测定血清中布比卡因对映体的在线联用高效液相色谱系统。该方法包括三个步骤：(i) 预浓缩和净化；(ii) 在反相柱上测定外消旋体；(iii) 将从反相柱上中心切割得到的外消旋体在α-糖蛋白柱上进行分离。该方法适用于测定血清中低至0.1微克/毫升的对映体，并且可以完全自动化。将展示患者的布比卡因时间进程。
