Oyama Y, Hayashi A, Ueha T, Chikahisa L, Furukawa K
Division of Pharmacological Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Brain Res. 1993 Apr 30;610(1):172-5. doi: 10.1016/0006-8993(93)91234-j.
Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidized to be fluorescent by cellular oxidants. Of the organic Ca2+ antagonists used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 microM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage.
使用流式细胞仪和2',7'-二氯荧光素，在解离的哺乳动物神经元上评估有机钙拮抗剂对氧化代谢或活性氧形成的影响，2',7'-二氯荧光素被细胞氧化剂氧化后会发出荧光。在所使用的有机钙拮抗剂中，氟桂利嗪和硝苯地平在1微摩尔及以上浓度时会降低荧光强度，而维拉帕米和地尔硫䓬则不会，这表明氟桂利嗪和硝苯地平对缺血性脑损伤具有一种有利作用。
