Salzman N P, Psallidopoulos M, Prewett A B, O'Leary R
Laboratory of Molecular Retrovirology, Georgetown University School of Medicine, Washington D.C. 20007.
Clin Orthop Relat Res. 1993 Jul(292):384-90.
Processed bone from an AIDS patient was tested for the presence of the human immunodeficiency virus type 1 (HIV-1). The preliminary procedures used to process bone allografts included removal of adventitious material and two cycles of freeze thawing. Although infectious virus was readily observed in plasma and bone marrow cells taken at autopsy, no infectious virus was detected in processed bone fragments. However, by using the polymerase chain reaction procedure, the presence of proviral HIV DNA could be demonstrated in processed bone allografts from this donor. Whereas the best safeguard against transmission of HIV by allografts is rigorous criteria for the exclusion of seropositive individuals as donors, proper procedures for processing bone allografts can further reduce the possibility of HIV transmission by bone allografts in cases where tissue from an infected donor is collected and processed.
对一名艾滋病患者的处理过的骨头进行了1型人类免疫缺陷病毒（HIV-1）检测。用于处理同种异体骨移植的初步程序包括去除外来物质和两个冻融循环。尽管在尸检时采集的血浆和骨髓细胞中很容易观察到感染性病毒，但在处理过的骨碎片中未检测到感染性病毒。然而，通过使用聚合酶链反应程序，可以证明来自该供体的处理过的同种异体骨移植中存在前病毒HIV DNA。虽然防止同种异体移植传播HIV的最佳保障措施是严格排除血清反应阳性个体作为供体的标准，但在收集和处理来自感染供体的组织的情况下，处理同种异体骨移植的适当程序可以进一步降低通过同种异体骨移植传播HIV的可能性。
