通过DNA指纹图谱鉴定近交系小鼠及其品系间的遗传关系。

Identification of inbred strains of mice and genetic relationships between strains as assessed by DNA fingerprinting.

作者信息

Mannen H, Tsuji S, Goto N, Fukuta K

机构信息

Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University, Hyogo, Japan.

出版信息

Jikken Dobutsu. 1993 Apr;42(2):169-73. doi: 10.1538/expanim1978.42.2_169.

DOI:10.1538/expanim1978.42.2_169

PMID:8519291

Abstract

Strain identification and the assessment of genetic relationships among inbred strains of mice were examined by DNA fingerprinting using M13 phage DNA as a probe. We used 8 inbred strains (NC/Jah, DDD/Jah, DSD/Jah, RR/Jah, SS/Jah, C3H/HeJah, C57BL/6 Jah and C57BL/6CrSlc) and coisogenic (NC-brp) as well as congenic (C57BL/6-bg) strains. DNA fingerprints using M13 phage DNA as a probe revealed identical patterns within strains. Bands due to new mutations in minisatellite regions within each strain were not observed. The banding patterns were strain-specific. Thus, DNA fingerprinting seemed to be suitable for genetic monitoring of mice. A dendrogram constructed with difference values calculated from DNA fingerprint patterns showed that relationships among strains reflected the history of these strains, so DNA fingerprinting could also be effectively used to assess the relationships between inbred strains of mice.

摘要

利用M13噬菌体DNA作为探针，通过DNA指纹图谱技术对近交系小鼠进行品系鉴定及遗传关系评估。我们使用了8个近交系（NC/Jah、DDD/Jah、DSD/Jah、RR/Jah、SS/Jah、C3H/HeJah、C57BL/6 Jah和C57BL/6CrSlc）以及同源近交系（NC-brp）和同源导入系（C57BL/6-bg）。以M13噬菌体DNA为探针的DNA指纹图谱显示各品系内图谱相同。未观察到各品系内小卫星区域新突变产生的条带。条带模式具有品系特异性。因此，DNA指纹图谱似乎适用于小鼠的遗传监测。根据DNA指纹图谱计算的差异值构建的树状图表明，品系间的关系反映了这些品系的历史，所以DNA指纹图谱也可有效地用于评估近交系小鼠之间的关系。