Sudo T, Goto N, Mannen H, Tsuji S, Fukuta K, Nishimura M
Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kobe University, Japan.
Exp Anim. 1995 Apr;44(2):87-93. doi: 10.1538/expanim.44.87.
SMXA recombinant inbred (RI) strains of mice were produced by systematic inbreeding from the F2 generation of a cross between two progenitor inbred strains, A/J and SM/J, which differ considerably with respect to many characteristics, and consists of 28 inbred strains. In this study, we investigated the applicability of DNA fingerprinting with M13 phage DNA to the identification of these closely related strains. DNA fingerprints of the SMXA RI strains and their progenitors, SM/J and A/J, showed strain-specific patterns, with the same banding patterns within each strain. Linkage analysis by using strain distribution patterns of minisatellite loci with 108 genetic markers containing microsatellites, biochemical and immunological marker genes allowed 23 minisatellite loci to be assigned to 11 chromosomes. The results suggested that DNA fingerprinting with M13 phage DNA is applicable not only for strain identification but also for genetic monitoring of RI strains on almost all chromosomes.
SMXA重组近交（RI）系小鼠是通过对两个祖系近交系A/J和SM/J杂交的F2代进行系统近交培育而成的。这两个祖系在许多特征上有很大差异，该重组近交系由28个近交系组成。在本研究中，我们调查了用M13噬菌体DNA进行DNA指纹分析在鉴定这些密切相关品系中的适用性。SMXA RI系及其祖系SM/J和A/J的DNA指纹显示出菌株特异性模式，每个菌株内具有相同的条带模式。通过使用包含微卫星、生化和免疫标记基因的108个遗传标记的小卫星位点的菌株分布模式进行连锁分析，可将23个小卫星位点定位到11条染色体上。结果表明，用M13噬菌体DNA进行DNA指纹分析不仅适用于品系鉴定，也适用于几乎所有染色体上RI系的遗传监测。
