Sasaki Y, Nonaka K, Nakata M
Department of Pediatric Dentistry, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Jikken Dobutsu. 1993 Apr;42(2):189-95. doi: 10.1538/expanim1978.42.2_189.
The embryo transfer technique is a very powerful tool for detecting intra-uterine effects of dams on prenatal growth of embryos. Embryos of DDD strain were transferred to a recipient of the same strain. Litter size, gestation period and birth weight of newborn offspring were measured. To examine the effect of embryo transfer on the prenatal growth of embryos, these results were compared with those of newborn DDD strain of mice that had developed from non-transferred embryos. Embryos of DDD strain were transferred to four strains of recipients (DDD/Qdj, C3H/Qdj, C57BL/Qdj and DBA/1J Sea). Litter size, gestation period and birth weight of newborn offspring were measured. The four strains of recipients were compared to examine the effect of recipient strain on the prenatal growth of the embryos. The results were as follows. 1. There was no gender difference in birth weight of offspring. 2. A negative correlation (partial regression coefficients: -0.024, P < 0.001 in the transfer and non-transfer groups, and -0.058, P < 0.001, in the four strains of recipients) was observed between litter size and birth weight of newborn offspring, while a positive correlation (partial regression coefficients: 0.068, P < 0.001 in the transfer and non-transfer groups, and 0.071, P < 0.001 in the four strains of recipients) was observed between gestation period and birth weight of newborn offspring. 3. Embryo transfer might affect the intra-uterine growth of transferred embryos. 4. The four different strains of recipients might contribute unequally to the prenatal growth of transferred embryos because of their different intra-uterine conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
胚胎移植技术是检测母鼠子宫内环境对胚胎产前生长影响的一种非常有效的工具。将DDD品系的胚胎移植到同品系的受体母鼠体内。测量新生仔鼠的窝仔数、妊娠期和出生体重。为了研究胚胎移植对胚胎产前生长的影响，将这些结果与未移植胚胎发育而来的DDD品系新生小鼠的结果进行比较。将DDD品系的胚胎移植到四种受体品系（DDD/Qdj、C3H/Qdj、C57BL/Qdj和DBA/1J Sea）的母鼠体内。测量新生仔鼠的窝仔数、妊娠期和出生体重。比较这四种受体品系，以研究受体品系对胚胎产前生长的影响。结果如下：1. 仔鼠出生体重不存在性别差异。2. 观察到新生仔鼠的窝仔数与出生体重呈负相关（移植组和未移植组的偏回归系数分别为-0.024，P<0.001；四种受体品系的偏回归系数为-0.058，P<0.001），而妊娠期与出生体重呈正相关（移植组和未移植组的偏回归系数分别为0.068，P<0.001；四种受体品系的偏回归系数为0.071，P<0.001）。3. 胚胎移植可能会影响移植胚胎在子宫内的生长。4. 由于子宫内环境不同，四种不同品系的受体对移植胚胎产前生长的影响可能不同。（摘要截选至250字）
