Koyama H, Wada T, Nishizawa Y, Iwanaga T, Aoki Y
Cancer. 1977 Apr;39(4):1403-9. doi: 10.1002/1097-0142(197704)39:4<1403::aid-cncr2820390408>3.0.co;2-8.
The effect of cyclophosphamide (CY) on ovarian function was studied in patients with breast cancer receiving prolonged daily administration of this agent (100 mg/day) after radical surgery. Out of 18 premenopausal patients that received 8.4-39.9 g CY, 15 developed permanent amenorrhea. The average dose given before the onset of amenorrhea was 5.2 g in patients in their 40s and 9.3 in their 30s. Urinary estrogens and serum progesterone were measured weekly for approximately 6 months postoperatively in six patients receiving CY. After the onset of amenorrhea, the levels of both hormones ceased to show their normal cyclic changes and remained low persistently, meanwhile serum FSH and LH were markedly elevated. No ovarian follicle was histologically found in three amenorrheic patients who underwent therapeutic oophorectomy after CY therapy. These findings indicate that CY induced primary ovarian failure.
研究了环磷酰胺（CY）对接受根治性手术后长期每日服用该药物（100毫克/天）的乳腺癌患者卵巢功能的影响。在18例接受8.4 - 39.9克CY的绝经前患者中，15例出现永久性闭经。40多岁患者闭经前的平均给药剂量为5.2克，30多岁患者为9.3克。对6例接受CY治疗的患者术后约6个月每周测量尿雌激素和血清孕酮。闭经后，两种激素水平均不再呈现正常的周期性变化，持续保持在低水平，同时血清促卵泡生成素（FSH）和促黄体生成素（LH）显著升高。在接受CY治疗后接受治疗性卵巢切除术的3例闭经患者中，组织学检查未发现卵巢卵泡。这些发现表明CY诱导了原发性卵巢功能衰竭。
