Ludvig N, Altura B T, Fox S E, Altura B M
Department of Physiology, State University of New York, Brooklyn 11203, USA.
Alcohol. 1995 Sep-Oct;12(5):417-21. doi: 10.1016/0741-8329(95)00012-g.
Intrahippocampal microdialysis was performed on 14 freely behaving rats, and the firing of pyramidal cells within the dialysis area was recorded. In one group of rats, the microdialysis was conducted only with artificial cerebrospinal fluid (ACSF) for 2-4 h. In this control group, the recorded neurons displayed normal firing patterns. In another group, ACSF was replaced for 30-60 min with various concentrations of ethanol to deliver this drug via the microdialysis probe into the cell recording area. Ethanol at the concentration of 5% (w/v) significantly and reversibly suppressed the firing of the recorded neurons. The marked firing rate alterations were not accompanied with apparent changes in the hippocampal EEG activity or the behavior of the rats, indicating localized drug actions. These data demonstrate for the first time that in the physiologically functioning brain, ethanol exerts principally a suppressant effect on the electrical activity of hippocampal pyramidal cells.
对14只自由活动的大鼠进行海马体内微透析，并记录透析区域内锥体细胞的放电情况。在一组大鼠中，仅用人工脑脊液（ACSF）进行2 - 4小时的微透析。在这个对照组中，记录的神经元表现出正常的放电模式。在另一组中，用不同浓度的乙醇替代ACSF 30 - 60分钟，通过微透析探针将这种药物输送到细胞记录区域。5%（w/v）浓度的乙醇显著且可逆地抑制了记录神经元的放电。明显的放电率变化并未伴随着海马脑电活动或大鼠行为的明显改变，表明药物作用具有局部性。这些数据首次证明，在生理功能正常的大脑中，乙醇主要对海马锥体细胞的电活动产生抑制作用。
